Determination of Microcystin in Water by High Performance Liquid Chromatography

Through comparison among some key links in the pre-treatment processes,a practical and convenient on-line determination method of microcystin by solid phase extraction/high performance liquid chromatography/ultraviolet was established.There was a linear relationship between the peak area of high performance liquid chromatography and the injection concentration of microcystin in the range of 0 to 1.8 mg/L.The method detection limit was 0.01 to 0.05 μg/L.After the extraction conditions were optimized,the method was used to determine microcystin in Microcystis aeruginosa.The recovery of three isomers was over 94%.