Human amnion-derived mesenchymal stem cells engraftment and differentiate in rats with acute myocardial infarction

Objective To observe the distribution and differentiation of hAD-MSCs injected intravenously and the feasibility of it used to repair the infracted heart.Methods SD rats were randomly assigned into hAD-MSCs transplanted,model and sham operation groups(12 rats each group).The hAD-MSCs were isolated from human amnion and then its phenotype was identified by flow cytometry and immunohistochemical staining.hAD-MSCs were injected into the subglossal vein of the SD rats 7 days after myocardial infarction(MI) was created and the organs such as the heart,liver and spleen were harvested at 6 weeks after transplantation.In order to assess the survival and differentiation of hAD-MSCs posttransplantation in the MI zone,as well as the influence on cardiac function.Results The isolated hAD-MSCs had the typical features of mesenchymal stem cells(MSCs),high expression of CD44,CD73,CD90,CD105 and vimentin.After injection through the subglossal vein,the hAD-MSCs passed through the capillary of the lung,and then distributed into the heart,lung and spleen,but seldom in the liver.At 6 weeks after hAD-MSCs transplantation,cell engraftments expressed cardiac-specific protein connexin-43 and α-actinin.The left ventricle function did not exacerbate after injection of hAD-MSCs compared with that in control group.Conclusions hAD-MSCs injected intravenously can distribute into myocardium,lung and spleen.The migration of injected cells delays the left ventricle remodeling after MI.