2007•Shandong Nongye Daxue xuebaoRequires access
ESTABLISHMENT AND IDENTIFICATION OF AN IN VITRO MURINE CARCINOMA CELL LINE WITH HBX GENE EXPRESSION
Pei Dong-sheng
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Abstract
To establish in vitro cell line with stable and effective HBxAg expression was established in order study to carcinogenesis mechanism of HBX gene and HBxAg.A recombinant plasmid(pcDNA3.1-HBX) containing the full length of HBX gene was transfected into EL4 cells.The expression of HBx in the cells was analyzed by Western blot using anti-HBx antibodies.Cell clones were obtained after being transfected with pcDNA3.1-HBX plasmid.Stable expression of HBxAg was detected by Western blot and RT-PCR.AHBX gene expression cell line was constructed in this study.The property of this system ensures stable and high level expression of HBx.It is a useful tool for functional studies of HBX gene.