2010•Zhongguo redai yixueRequires access
Establishment and identification of in vitro HL7702 and HepG2 cell ines with HBX gene expression.
Hongjuan You, Pei Dong-sheng, Xiaomei Liu, Renxian Tang
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Abstract
Aim To establish in vitro cell line with stable and effective HBxAg expression,for study of carcinogenesis mechanism of HBX gene and HBxAg.Methods A recombinant plasmid (pcDNA3.1-HBX)containing the full length of HBX gene was transfected into HL7702 cells and HepG2 cells.The expression of HBx in the cells was analyzed by Western blot and immunofluorescency using anti-HBx antibodies.Results Cell clones were obtained after being transfected with pcDNA3.1-HBX plasmid.Stable expression of HBxAg was detected by Western blot,immunofluorescency and RT-PCR.Conclusion HBX gene expression cell lines were constructed in this study.The property of this system ensures stable and high level expression of HBx.It is a useful tool for functional studies of HBX gene.