Two different postconditioning measure on focal cerebralischemia-reperfusion injury in rats

Objective To investigate the effects of combination of ischemic postconditioning and limb ischemic postconditioning on focal cerebral ischemia-reperfusion injury in rats.Methods A total of 80 SD rats were randomly divided into 5 groups:Group sham operation(GroupⅠ),Group ischemia reperfusion(GroupⅡ),Group ischemic postconditioning(GroupⅢ),Group limb ischemic postconditioning(GroupⅣ)and Group combined postconditioning(GroupⅤ).The model of focal cerebral ischemia-reperfusion was induced by the method of thread embolism.The neurologic deficit scores were obtained after 24 h of reperfusion.The rats were decapitated and brains were removed rapidly for determination of the cerebral infarct size,malondialdehyde(MDA)content,superoxide dismutase(SOD)activities and tumor necrosis factor alpha(TNF-α)level.Results The size of cerebral infarct were 0,(55.29±16.34)%,(36.07±14.76)%,(37.23±15.13)% and (21.15±10.92)%;Compared with GroupⅠ,the scores of neurologic deficit,the level of TNF-α and the content of MDA were significantly increased,while the activities of SOD were significantly decreased in the other 4 groups(all P0.05).Compared with group Ⅱ,the activities of SOD were increased,while other indexs decreased in the Groups Ⅲ,Ⅳ and Ⅴ(all P0.05).Compared with group Ⅴ,the activities of SOD were decreased,while other indexs increased in the Groups Ⅲ and Ⅳ(all P0.05).The indexs were not different among Group Ⅲ and Ⅳ(all P0.05).Conclusions The combination of ischemic postconditioning and limb ischemic postconditioning can attenuate the injury of focal cerebral ischemia-reperfusion in rats.And the treatment combined displays synergistic effect.