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Simultaneous determination of osthole and isopimpinellin in Fructus Cnidii by HPLC

Baojin Chen

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Abstract

Objective To establish an HPLC method for the simultaneous determination of osthole and isopimpinellin in Fructus Cnidii.Methods HPLC analysis was performed on a Promosil C18(250 mm×4.6 mm,5 μm) column.The column temperature was 30 ℃.The mobile phase was a mixed liquid with MeOH-0.1% H3PO4(80∶20) and the flow rate was 0.8 mL·min-1.The detection wavelength was 265 nm.Results A good linearity of osthole was at 0.2191~2.191 μg,and the average recovery was 98.15%.A good linearity of isopimpinellin was at 0.0126~0.126 μg,and the average recovery was 99.23%.Conclusion The method is simple,rapid,accurate and reproducible for the simultaneous determination of osthole and isopimpinellin in Fructus Cnidii.

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What this paper is about

Objective To establish an HPLC method for the simultaneous determination of osthole and isopimpinellin in Fructus Cnidii.Methods HPLC analysis was performed on a Promosil C18(250 mm×4.6 mm,5 μm) column.The column temperature was 30 ℃.The mobile phase was a mixed liquid with MeOH-0.1% H3PO4(80∶20) and the flow rate was 0.8 mL·min-1.The detection wavelength was 265 nm.Results A good linearity of osthole was at 0.2191~2.191 μg,and the average recovery was 98.15%.A good linearity of isopimpinellin was at 0.0126~0.126 μg,and the average recovery was 99.23%.Conclusion The method is simple,rapid,accurate and reproducible for the simultaneous determination of osthole and isopimpinellin in Fructus Cnidii.

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Available abstract

Objective To establish an HPLC method for the simultaneous determination of osthole and isopimpinellin in Fructus Cnidii.Methods HPLC analysis was performed on a Promosil C18(250 mm×4.6 mm,5 μm) column.The column temperature was 30 ℃.The mobile phase was a mixed liquid with MeOH-0.1% H3PO4(80∶20) and the flow rate was 0.8 mL·min-1.The detection wavelength was 265 nm.Results A good linearity of osthole was at 0.2191~2.191 μg,and the average recovery was 98.15%.A good linearity of isopimpinellin was at 0.0126~0.126 μg,and the average recovery was 99.23%.Conclusion The method is simple,rapid,accurate and reproducible for the simultaneous determination of osthole and isopimpinellin in Fructus Cnidii.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry

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Simultaneous determination of osthole and isopimpinellin in Fructus Cnidii by HPLC — Research Paper | ScholarLens