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RP-HPLC Determination of Osthole in Fructus Cnidii

Wu Bo

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Abstract

Objective:To develop a quantitative method for determination of osthole in Fructus Cnidii by RP-HPLC. Method:BDSHYPERSIL C18 column(250 mm×4.6 mm,5μm) was used.ethanol-water(60:40) as the mobile phase,flow rate at 1 mL·min-1, fluorescence detector:λex357 nm,λex395 nm. Results:The good linear relationship was obtained under the optimum conditions. The recoveries of the standards added was 100.6% while the relative standard deviations was 1.8%.Conclusions:The method is rapid and precise.

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What this paper is about

Objective:To develop a quantitative method for determination of osthole in Fructus Cnidii by RP-HPLC. Method:BDSHYPERSIL C18 column(250 mm×4.6 mm,5μm) was used.ethanol-water(60:40) as the mobile phase,flow rate at 1 mL·min-1, fluorescence detector:λex357 nm,λex395 nm. Results:The good linear relationship was obtained under the optimum conditions. The recoveries of the standards added was 100.6% while the relative standard deviations was 1.8%.Conclusions:The method is rapid and precise.

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Available abstract

Objective:To develop a quantitative method for determination of osthole in Fructus Cnidii by RP-HPLC. Method:BDSHYPERSIL C18 column(250 mm×4.6 mm,5μm) was used.ethanol-water(60:40) as the mobile phase,flow rate at 1 mL·min-1, fluorescence detector:λex357 nm,λex395 nm. Results:The good linear relationship was obtained under the optimum conditions. The recoveries of the standards added was 100.6% while the relative standard deviations was 1.8%.Conclusions:The method is rapid and precise.

Key concepts: Chemistry, Chromatography, High-performance liquid chromatography, Relative standard deviation, Quantitative analysis (chemistry), Volumetric flow rate, Analytical Chemistry (journal), Detection limit

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