Study on Chemical Constituents from Tibetan Herb Pyrethrumta tsienense

Objective To study the chemical constituents of Tibetan herb Pyrethrumta tsienense(Bur. et Franch.)Ling,thus to supply evidence for its pharmacological research and therapeutic mechanism. Methods The components of methanol extract of Pyrethrumta tsienense(Bur. et Franch.)Ling was isolated and purified by normal phase silica gel,reversed phase silica gel and Sephadex LH- 20. The structures of compounds were identified by spectral analysis of mass spectrum and NMR(1H- NMR,13C NMR)spectrum. Results Fourteen flavonoids were obtained from ethyl acetate fraction and n- butanol fraction,and they were tricin(1),4,- methoxy- tricin(2),apiolin(3),luteolin(4),quercetin(5),axillarin(6),luteolin- 7- O- β- D- glucoside(7),apiolin- 7- O- β- D- glucoside(8),apigenin- 7- O- β-D- glucuronic acid methyl ester(9),quercetin- 3- O- β- D- glucoside(10),6- hydroxy- luteolin- 7- O- β- D- glucoside(11),luteolin- 7- O- β- D- glucoside aid(12),scopoletin- 7- O- β- D- glucoside aid(13),and chrysoeriol- 7- O-β- D- glucoside aid(14). Conclusion Compounds 8- 14 have been isolated from the Pyrethrumta genus for the first time.