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2010•Acta Pratacultural ScienceRequires access

Genetic diversity of wild Cynodon dactylon germplasm detected by AFLP markers.

Xiaofang Qi, Zhang XinQuan, Ling Yao, Shiyong Chen, Wei Liu

Open publisher page 2 citations

Abstract

Amplified fragment length polymorphism(AFLP)molecular markers were applied to detect the genetic variation of 44 agrestal accessions of Cynodon dactylon collected from Africa,Sichuan,Chongqing,Yunnan,Guizhou,and Tibet in China.A total of 462 bands were amplified by ten AFLP primer pairs from C.dactylon genetic DNA,of which 452 (97.64%) bands were found to be polymorphic.The AFLP-based genetic similarity values among 44 C.dactylon accessions ranged from 0.64 to 0.95,and the average Nei’s coefficient was 0.76.Analysis of cluster and principal component analysis showed that all the accessions could be distinguished by AFLP markers and divided into 5 groups.Accessions from the same area were almost all classified into the same group associated with their geographical distributions.Therefore,complex geographical ecological environments are important factors for genetic diversity and geographical distribution of C.dactylon.

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What this paper is about

Amplified fragment length polymorphism(AFLP)molecular markers were applied to detect the genetic variation of 44 agrestal accessions of Cynodon dactylon collected from Africa,Sichuan,Chongqing,Yunnan,Guizhou,and Tibet in China.A total of 462 bands were amplified by ten AFLP primer pairs from C.dactylon genetic DNA,of which 452 (97.64%) bands were found to be polymorphic.The AFLP-based genetic similarity values among 44 C.dactylon accessions ranged from 0.64 to 0.95,and the average Nei’s coefficient was 0.76.Analysis of cluster and principal component analysis showed that all the accessions could be distinguished by AFLP markers and divided into 5 groups.Accessions from the same area were almost all classified into the same group associated with their geographical distributions.Therefore,complex geographical ecological environments are important factors for genetic diversity and geographical distribution of C.dactylon.

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Available abstract

Amplified fragment length polymorphism(AFLP)molecular markers were applied to detect the genetic variation of 44 agrestal accessions of Cynodon dactylon collected from Africa,Sichuan,Chongqing,Yunnan,Guizhou,and Tibet in China.A total of 462 bands were amplified by ten AFLP primer pairs from C.dactylon genetic DNA,of which 452 (97.64%) bands were found to be polymorphic.The AFLP-based genetic similarity values among 44 C.dactylon accessions ranged from 0.64 to 0.95,and the average Nei’s coefficient was 0.76.Analysis of cluster and principal component analysis showed that all the accessions could be distinguished by AFLP markers and divided into 5 groups.Accessions from the same area were almost all classified into the same group associated with their geographical distributions.Therefore,complex geographical ecological environments are important factors for genetic diversity and geographical distribution of C.dactylon.

Key concepts: Cynodon dactylon, Amplified fragment length polymorphism, Genetic diversity, Germplasm, Biology, Genetic variation, Botany, Genetic distance

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