Screening Differential Expression of Taxol-resistance Related Gene of Ovarian Carcinnoma by cDNA Microarray

Objective To study the gene differential expression of taxol resistance cell line OC3/Tax300 of ovarian carcinnoma compared with other parent cell OC3,screen drug-resistance related gene by cDNA microarray, and discuss the reiationship between gene expression difference and drug-resistance.Methods The cDNA retro-transcribed from equal quantity mRNA derived from OC3/Tax300 and OC3 which were labeled with Cy5 and Cy3 fluorescence as probe respectively. The mixed probe were hybridized with BiostarH140S gene expression chip. The acquired image was analyzed by software.Results 134 signitificantly differently expressed genes were screened out, of which up-and down regulated genes were 17 and 117 respectively. They were related to 14 kinds of genes. The down-regulated genes were EBNA-3,COP9, StIP1,and so on. The up-regulated genes were JAK2,HSPS,NADH, and so on.Conclusion These genes related to the mechnisms of drug-resistance of ovarian cancer.