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Study of the drug-resistance related genes of taxolin patients with ovarian carcinoma

Lei Song

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Abstract

Objective: To study the gene differential expression of taxol resistance cell line OC3/Tax300 of ovarian carcinoma compared with other parent cell OC3 and to screen drug-resistance related gene by cDNA microarray.Methods: The cDNA retro-transcribed from equal quantity mRNA derived from OC3/Tax300 and OC3 which were labeled with Cy5 and Cy3 fluorescence as probe respectively.The mixedprobe were hybridized with BiostarH140S gene expression chip.The acquired image was analyzed by software.Results: 234 signitificantly differently expressed genes were screened out,of which up-and down-regulated genes were 17 and 217respectively.They were related to 14 kinds of genes.The down-regulated genes were EBNA-3,COP9,StIP1,and so on.The up-regulated genes were JAK2,HSPs, NADH,and so on.Conclusion: These genes may be related to the mechnisms of drug-resistance of ovarian cancer.

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What this paper is about

Objective: To study the gene differential expression of taxol resistance cell line OC3/Tax300 of ovarian carcinoma compared with other parent cell OC3 and to screen drug-resistance related gene by cDNA microarray.Methods: The cDNA retro-transcribed from equal quantity mRNA derived from OC3/Tax300 and OC3 which were labeled with Cy5 and Cy3 fluorescence as probe respectively.The mixedprobe were hybridized with BiostarH140S gene expression chip.The acquired image was analyzed by software.Results: 234 signitificantly differently expressed genes were screened out,of which up-and down-regulated genes were 17 and 217respectively.They were related to 14 kinds of genes.The down-regulated genes were EBNA-3,COP9,StIP1,and so on.The up-regulated genes were JAK2,HSPs, NADH,and so on.Conclusion: These genes may be related to the mechnisms of drug-resistance of ovarian cancer.

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Available abstract

Objective: To study the gene differential expression of taxol resistance cell line OC3/Tax300 of ovarian carcinoma compared with other parent cell OC3 and to screen drug-resistance related gene by cDNA microarray.Methods: The cDNA retro-transcribed from equal quantity mRNA derived from OC3/Tax300 and OC3 which were labeled with Cy5 and Cy3 fluorescence as probe respectively.The mixedprobe were hybridized with BiostarH140S gene expression chip.The acquired image was analyzed by software.Results: 234 signitificantly differently expressed genes were screened out,of which up-and down-regulated genes were 17 and 217respectively.They were related to 14 kinds of genes.The down-regulated genes were EBNA-3,COP9,StIP1,and so on.The up-regulated genes were JAK2,HSPs, NADH,and so on.Conclusion: These genes may be related to the mechnisms of drug-resistance of ovarian cancer.

Key concepts: Gene, Complementary DNA, Ovarian carcinoma, Microarray, Biology, Gene expression, Drug resistance, Ovarian cancer

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