Regulation of Integrin α_2,α_5,β_1 mRNA Expression on Osteoblasts Membrane by bFGF

Objective To observe the regulation of bFGF on mRNA expression of integrin subunits α2,α5,β1 in osteoblasts.Methods Osteoblasts were pre-cultured for 24 h with F12 medium containing bFGF with different concentration and planted on the sand blasting titanium disks.Three days after planting,osteoblasts were harvested.The mRNA expression of integrin subunit α2,α5,β1 were examined by immunofluorescent quantitative real-time polymerase chain reaction(RT-PCR).Results The mRNA expression of integrin β1 is most abundant and the mRNA expressions of integrin α2,α5 are relatively lower than integrin β1.In the study,the mRNA expression levels of integrin α2,α5 and β1 were up-regulated after bFGF stimulation.However,the effective concentration range of bFGF to the three subunits was different.Conclusion bFGF could up-regulate the mRNA expression of integrin subunits in osteoblasts.