2009•Chinese Journal of Otorhinolaryngology-skull Base SurgeryRequires access

Study of apoptosis of CNE-2 cells of nasopharyngeal carcinoma induced by sinoactinomycin

Lin Chang

Abstract

Objective To determine the apoptotic effect and its mechanism of sinoactinomycin on the human CNE-2 cells of nasopharyngeal carcinoma in vitro.Methods CNE-2 cells were treated with sinoactinomycin at different concentrations.The growth inhibition effect of sinoactinomycin was determined by MTT assay.Apoptosis was evaluated under inverted microscope.Bax mRNA expression was detected by reverse transcriptase-polymerase chain reaction(RT-PCR).Results Sinoactinomycin significantly inhibited the growth of the CNE-2 cells.The Bax mRNA level was significantly increased in sinoactinomycin treated cells compared to non-treated cells in a concentration dependant manner.Conclusion Sinoactinomycin inhibits the growth of CNE-2 cells due to apoptosis induced by the up-regulatoin of Bax.

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Key concepts: Nasopharyngeal carcinoma, Apoptosis, Messenger RNA, In vitro, Molecular biology, MTT assay, Chemistry, Growth inhibition