Studied on Resveratrol in Inducing apoptosis of Nasopharyngeal Carcinoma CNE-1 Cells and Its Molecular Mechanism

Objective To elucidate the effects of resveratrol on inducing apoptosis of nasopharyngeal carcinoma CNE- 1 cells and its molecular mechanism. Methods The viability of CNE- 1 cells was detected by methyl thiazolyl tetrazolium(MTT)assay,cell cycle profile was analyzed by PI single- staining flow cytometer and apoptosis of CNE- 1cells was analyzed by PI- AnnexinV double- staining flow cytometer. The expression level of target proteins was examined by immunoblotting method. Results Resveratrol significantly inhibited the viability of CNE- 1 cells in concentration- and time- dependent manner(P 0.01 compared with the normal control group). Treatment with resveratrol resulted in the increased the percentage of S- phase CNE- 1 cells in dose- dependent manner. Accordingly, Cyclin E and Cyclin A were downregulated in resveratrol- treated CNE- 1 cells. Furthermore, resveratrol dose- dependently induced the apoptosis of CNE- 1 cells. Correspondingly, the anti- apoptotic protein Bcl- 2 was downregulated and the pro- apoptotic protein Bax was upregulated in CNE- 1 cells after treatment with resveratrol. Conclusion Resveratrol can arrest CNE- 1 cells at S phase via downregulating the expression of cyclinE and Cyclin A, and can further induce apoptosis through mitochondrial- mediated intrinsic cell- death pathways.