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2004•Journal of Apoplexy and Nervous DiseasesRequires access

The effect of human nerve growth factor on CGTmRNA in sciatic nervers of experimental diabetic polyneuropathy rats

Jiang Yu-ping

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Abstract

Objective To observe the effect of human nerve growth factor(hNGF) on UDP-Galactose:Ceramide Galactosyltransferase mRNA (CGTmRNA) in sciatic nervers of experimental diabetic polyneuropathy rats.Methods Diabetic rat was induced by a single intraperitoneal injection of streptozotocin.After 4 weeks of diabetes,hNGF(90U/kg/d) was injected intraperitoneal for 4weeks.The level of CGTmRNA,NGFmRNA and protein expressions in sciatic nerve were measured by RT-PCR and immunohistochemistry techniques.Results After 8 weeks of diabetes,CGTmRNA expression was increased significantly, NGFmRNA and protein levels were decreased in sciatic nerves.After treatment withhNGF for 4 weeks,CGTmRNA expression was decreased,and NGFmRNA and protein levels wereincreased.Conclusion Administration of hNGF can reverse abnormal expression of CGTmRNA in sciatic nervers of experimental diabetic polyneuropathy rats.

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What this paper is about

Objective To observe the effect of human nerve growth factor(hNGF) on UDP-Galactose:Ceramide Galactosyltransferase mRNA (CGTmRNA) in sciatic nervers of experimental diabetic polyneuropathy rats.Methods Diabetic rat was induced by a single intraperitoneal injection of streptozotocin.After 4 weeks of diabetes,hNGF(90U/kg/d) was injected intraperitoneal for 4weeks.The level of CGTmRNA,NGFmRNA and protein expressions in sciatic nerve were measured by RT-PCR and immunohistochemistry techniques.Results After 8 weeks of diabetes,CGTmRNA expression was increased significantly, NGFmRNA and protein levels were decreased in sciatic nerves.After treatment withhNGF for 4 weeks,CGTmRNA expression was decreased,and NGFmRNA and protein levels wereincreased.Conclusion Administration of hNGF can reverse abnormal expression of CGTmRNA in sciatic nervers of experimental diabetic polyneuropathy rats.

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Available abstract

Objective To observe the effect of human nerve growth factor(hNGF) on UDP-Galactose:Ceramide Galactosyltransferase mRNA (CGTmRNA) in sciatic nervers of experimental diabetic polyneuropathy rats.Methods Diabetic rat was induced by a single intraperitoneal injection of streptozotocin.After 4 weeks of diabetes,hNGF(90U/kg/d) was injected intraperitoneal for 4weeks.The level of CGTmRNA,NGFmRNA and protein expressions in sciatic nerve were measured by RT-PCR and immunohistochemistry techniques.Results After 8 weeks of diabetes,CGTmRNA expression was increased significantly, NGFmRNA and protein levels were decreased in sciatic nerves.After treatment withhNGF for 4 weeks,CGTmRNA expression was decreased,and NGFmRNA and protein levels wereincreased.Conclusion Administration of hNGF can reverse abnormal expression of CGTmRNA in sciatic nervers of experimental diabetic polyneuropathy rats.

Key concepts: Sciatic nerve, Intraperitoneal injection, Streptozotocin, Medicine, Nerve growth factor, Polyneuropathy, Internal medicine, Endocrinology

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The effect of human nerve growth factor on CGTmRNA in sciatic nervers of experimental diabetic polyneuropathy rats — Research Paper | ScholarLens