Influence of salt curing on the infectivity of Trichinella spiralis muscle larvae in mice

Objective To observe the influence of salt curing on infectivity of Trichinella spiralis muscle larvae in mice.Methods Twenty female Kunming mice were randomly divided into one control group and three test groups,thus 4groups with 5mice per group in total.As to the control group,each mouse was inoculated orally with 300muscle larvae of T.spiralis.There are three test groups(salt curing groups).Group one's sample were salted 24h at 4℃;group two's sample were salted 48hat 4℃;and group three's sample were salted 72hat 4 ℃.Then,each mouse was orally inoculated with 300disposed muscle larval of T.spiralis.All infected mice were sacrificed on the 28th day after infection,and muscle larval of T.spiralis were examined respectively by diaphragma compression method and digestion method for all infected mice.Results Muscle larval detection rate by compression method and digestion method were 100%in all mice infected with the larval which were salted 24hand 48h.These two groups'averages were obviously lower than that of the control group(P 0.01).Group two(48h)'s average was obviously lower than that of the group one(24h)(P0.01).Group three(72h)'s infectivity was zero.Conclusion Salt does the vital harm to the muscle larval of T.spiralis.With curing time extending,encapsulated larvae's infection gradually decreases till finally disappears.