Experimental studies on influence of sausage curing on the infectivity of Trichinella spiralis muscle larvae in mice

Objective To observe the influence of sausage curing on infectivity of Trichinella spiralis(T.spiralis)muscle larvae in mice.Methods Thirty male Kunming mice were randomly divided into one control group and five test groups,thus 6groups with 5mice per group in total. As to the control group,each mouse was inoculated orally with 300muscle larvae of T.spiralis. There were five test groups(sausage curing groups).Five groups' samples were cured 24h,48h, 72h,96h,120hat 4℃.Then,each mouse was orally inoculated with 300disposed muscle larval of T.spiralis.All infected mice were sacrificed on the 28th day after infection,and muscle larval of T.spiralis were examined respectively by diaphragma compression method and digestion method for all infected mice.Results Muscle larval detection rate by compression method and digestion method were 100% in all mice infected with the larval which were cured 24h,48h,72h and 96h.These four groups' averages were obviously lower than that of the control group(P0.01).Group 48h,group 72hand group 96haverage were obviously lower than that of the group 24h(P0.01).Group 120hinfectivity was zero.Conclusion With curing time extending, encapsulated larvae's infection gradually decreases.