Inhibitory effects of lisinopril on obstructive cholestasis induced liver fibrosis in mice

Objective To study the effect of lisinopril on bile duct ligation-induced biliary cholestasis on liver of mice.Methods The experimental model mice of hepatic fibrosis was induced by common bile duct ligation,which were divided into model control group and lisinopril group.Three weeks after the operation,lisinopril(0.1 mg/kg) and physiological saline(10 ml/kg) were administrated respectively by intragastric in lisinopril group and model control group,with once a day for 4 consecutive weeks.False operation group was as blank control in the same period.All the mice were sacrificed six weeks later.Liver tissues of the three groups were detected by routine histological staining and collagen fiber staining;serum hyaluronate lyase(HA) and laminin(LN) levels were measured by radioimmunoassay,including liver hydroxyproline(HYP) content.Results Serum HA and LN contents in model control group and lisinopril group were decresed and liver HYP content were increased compared with those in blank control group(P0.05).Liver cells injury and the degree of liver fibrosis in lisinopril group were lighter compared with those in model group.Serum HA,LN contents and liver HYP content in lisinopril group were(62.1±2.23) ng/ml,(42.8±6.23) ng/ml and(1.6±0.150) μg/mg,significantly lower than those in model group [(97.5±4.50)ng/ml,(66.5±7.32) ng/ml and(2.0±0.27) μg/mg;all the P0.05].Conclusion Lisinopril has effect on preventing hepatic fibrosis in mice model with bile duct ligation-induced biliary cholestasis.