Protective effect of PF4 on bone marrow karyocytes from mice treated with cyclophosphamide

AIM To provide evidence for studying the chemoprotective effect of PF4 and its machanism on murine hematopoietic cells treated with CTX. METHODS The mice were intraperitoneal (IP) injected with PF4 (40 μg·kg -1 ) twice at 6 h intervals. 20 h after the second injection they were given one injection of CTX (200 mg·kg -1 ) to make the models of chemical lesions. Then the numbers of their peripheral WBC were observed. 5 d, 8 d later the DNA contents in bone marrow cells were detected by flow cytemetry (FCM). RESULTS There were no difference between the peripheral WBC counts of PF4 treatment groups and the one of CTX groups ( P 0.05). On day 5, bone marrow cells in G 0/G 1 phase in PF4 treatment groups were significantly higher than that in CTX groups, and the necrotic and apoptotic percentage were significantly lower, but there was no difference between the PF4 groups and the PBS control groups. On day 8 after CTX treatment, bone marrow cellularity in PF4 treatment groups were significantly higher than that in CTX groups. CONCLUSION PF4 can protect murine bone marrow from the cytotoxic effects of CTX. PF4 should be a potent protective agent during chemotherapy.