Effects of PF4 on bone marrow cell proliferative activity in mice with CTX-induced injury

Objective To explore the effects of PF4 on bone marrow cell proliferation in mice with CTX-induced injury. Methods Thirty-six BALB mice were randomly divided into PF4 group(n=12),CTX group(n=12)and control group(n=12).The mice were intraperitoneally injected with CTX 200 mg/kg to establish chemical lesion models.At hour 26 and 20 before administration of CTX,PF4(40 μ/kg)was injected in PF4 group,and PBS in CTX group.In control group,PBS was injected without CTX.The effects of PF4 on CFU-GM forming of CTX-treated mice were evaluated.CFU-GM was assayed using a double-layer agar culture system in vitro.The DNA contents in bone marrow cells were detected by flow cytometry(FCM). Results At day 5 after injecting CTX,the numbers of bone marrow cells in CTX group significantly decreased compared with control group(P0.01).At day 8,the numbers of bone marrow cells in PF4 groups were significantly higher than that in CTX group(P0.01).At day 5,bone marrow CFU-GM yields in CTX group significantly decreased compared with control group and PF4 group(P0.01).At day 8,CFU-GM yields in PF4 group and CTX group increased significantly,higher in PF4 group than in CTX group.At day 5,bone marrow cells in G0/G1 phase in PF4 group were significantly higher than that in CTX group,and the necrotic and apoptotic percentages were significantly lower(P0.01),but there was no significant difference between PF4 group and control group.At day 8,there was no significant difference among three groups(P0.05). Conclusion PF4 can increase bone marrow cells in G0/G1 phase,decrease the necrotic and apoptotic percentage,and increase bone marrow CFU-GM yields.