Study on Optimization of Fermentation Medium of Escherichia coli XD-12 for Aminotransferase Production by Response Surface Methodology

[Objective]The research aimed to optimize the fermentation medium of E.coli XD-12 for amiontransferase production by response surface methodology,to enhance the yield of L-phenylalanine.[Method]Based on the single factor test,fermentation medium of Escherichia coli XD-12 for aminotransferase production was optimized by response surface methodology.[Result]The results showed that the composition of the optimal medium was glucose 10.0 g/L,corn steep liquor 28.3 ml/L,peptone 9.3 g/L,MgSO_4 0.5 g/L,NaCl 0.5 g/L,pH value 7.2.Under this condition,the maximum yield of L-phenylalanine was 93.8%as the predictive value and 93.0%as the practical value.[Conclusion] The model can better predict the real fermentation situation,and it is feasible to be used to optimize the fermentation medium of E.coli.