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Imipenem-resistant Mechanism of Pseudomonas Aeruginosa

Zhang Yi-jie, Yanqiu Hu

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Abstract

OBJECTIVE:To investigate the mechanism of imipenem-resistance in clinical isolates of Pseudomonas aeruginos (PA). METHODS: 55 strains of PA had been isolated from sputum specimen in clinic and their drug sensitivity to imipenem was tested by K-B slip diffusion method. Their amount of outer membrane protein OprD2 was analyzed by SDS-PAGE with outer membrane protein profiles. Metallo-β-lactamases (MBLs) was detected by double-disk synergy test (DDST). RESULTS: 36 strains of imipenem-resistant and 19 strains of imipenem-sensitive PA isolated were studied. The amount of OprD2 showed a various degree of reduction in all resistant isolates, and the relative amount of OprD2 in resistant isolates were significantly lower than in sensitive isolates (P0.01). 6 (10.9%) were MBLs-producing strains. CONCLUSIONS: The reduction of OprD2 and MBLs are the main factors accountable for Pseudomonas aeruginosas's resistance to imipenem.

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OBJECTIVE:To investigate the mechanism of imipenem-resistance in clinical isolates of Pseudomonas aeruginos (PA). METHODS: 55 strains of PA had been isolated from sputum specimen in clinic and their drug sensitivity to imipenem was tested by K-B slip diffusion method. Their amount of outer membrane protein OprD2 was analyzed by SDS-PAGE with outer membrane protein profiles. Metallo-β-lactamases (MBLs) was detected by double-disk synergy test (DDST). RESULTS: 36 strains of imipenem-resistant and 19 strains of imipenem-sensitive PA isolated were studied. The amount of OprD2 showed a various degree of reduction in all resistant isolates, and the relative amount of OprD2 in resistant isolates were significantly lower than in sensitive isolates (P0.01). 6 (10.9%) were MBLs-producing strains. CONCLUSIONS: The reduction of OprD2 and MBLs are the main factors accountable for Pseudomonas aeruginosas's resistance to imipenem.

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Available abstract

OBJECTIVE:To investigate the mechanism of imipenem-resistance in clinical isolates of Pseudomonas aeruginos (PA). METHODS: 55 strains of PA had been isolated from sputum specimen in clinic and their drug sensitivity to imipenem was tested by K-B slip diffusion method. Their amount of outer membrane protein OprD2 was analyzed by SDS-PAGE with outer membrane protein profiles. Metallo-β-lactamases (MBLs) was detected by double-disk synergy test (DDST). RESULTS: 36 strains of imipenem-resistant and 19 strains of imipenem-sensitive PA isolated were studied. The amount of OprD2 showed a various degree of reduction in all resistant isolates, and the relative amount of OprD2 in resistant isolates were significantly lower than in sensitive isolates (P0.01). 6 (10.9%) were MBLs-producing strains. CONCLUSIONS: The reduction of OprD2 and MBLs are the main factors accountable for Pseudomonas aeruginosas's resistance to imipenem.

Key concepts: Imipenem, Microbiology, Pseudomonas aeruginosa, Pseudomonas, Drug resistance, Agar diffusion test, Sputum, Biology

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