Isolation, culture and purification of olfactory ensheathing cells from human fetal olfactory mucosa and from human fetal olfactory bulb

[Objective]To investigate differential adhesion method and immunohistochemistry in human embryo and human embryonic olfactory mucosa olfactory mucosal OECs OECs purified in vitro culture,explore the establishment of olfactory mucosa and olfactory bulb OECs OECs in vitro methods.[Method]The differential adhesion of human fetal olfactory mucosa after olfactory bulb OECs and OECs were alternatively contained DMEM-F12 13% FBS medium in primary culture.The morphological changes of olfactory ensheathing cells were observed,p75NTR and GFAP immunocytochemistry for identification and purity testing was used.[Result] The human embryo and human embryonic olfactory mucosa olfactory bulb could be cultivated olfactory ensheathing cells,olfactory mucosa olfactory ensheathing cells form mostly bipolar,tripolar,with slender processes.p75NTR and GFAP staining were positive reaction.In vitro the purity of human fetal olfactory ensheathing cells cultured from human fetal olfactory ensheathing cells was higher than from olfactory mucosa.[Conclusion]The differential adhesion method can be isolated and cultured human embryonic cells and human fetal olfactory mucosa olfactory ensheathing cells,olfactory bulb.