Role of Survivin Gene on the Apoptosis of Adenoid Cystic Carcinoma-M Cells Induced by Arsenic Trioxide

Objective: To investigate the proliferation inhibition of arsenic trioxide(As2O3) on salivary adenoid cystic carcinma-M(ACC-M) cells in vitro and to study the role of Survivin on the apoptosis of ACC-M induced by As2O3.Methods: ACC-M cells were treated with different concentration of As2O3 for different periods.The inhibitory effects on cell's growth were assayed with MTT test.Apoptosis was determined by flow cytometry.The expression of Survivin mRNA and protein were investigated by RT-PCR and Western blot analysis respectively.Results: Cell viability after As2O3 treatment was markedly suppressed and exhibited as a dose-and time-dependent pattern.The apoptotic index showed the similar trend.The results of RT-PCR revealed gene expression of Survivin was suppressed significantly.Through Western blot analysis,a negative correlation between concentration and amount of protein product of Survivin was determined.Conclusion: As2O3 might markedly suppressed ACC-M cell's viability in vitro.The inhibition of Survivin gene expression may play a critical role on ACC-M cell apoptosis induced by As2O3.