Effects of arsenic trioxide on the cell proliferation and expression of Survivin in human epidermis cancer cell line-A 431in vitro

Objective To explore the effects of arsenic trioxide(As2O3) on the cell proliferation and expression of Survivin in human epidermal carcinoma cell line-A 431 in vitro.Methods The human epidermal carcinoma cells(cell line A-431) were cultured in vitro,then the cells were treated with different concentrations of arsenic trioxide,then the effects of arsenic trioxide on the cell proliferation of A 431 were detected by MTT,the effects of arsenic trioxide on the cell cycle,apoptosis and expression of Survivin were measured by flow cytometry and the effects of arsenic trioxide on expression of Survivin were also tested by Western Blot.Results The As2O3 could obviously inhibit the proliferation of A 431 cells with concentrations from 10μmol/L to 40μmol/L,which was time-dependent and concentration-dependent.The As2O3 could affect the cell cycle of A 431cells,with the increase of cell counts at G0/G1 phase and the increase of apoptotic cell counts(P0.05).As compared with control group,As2O3 could significantly inhibit the expression of Survivin of A 431 cells.Conclusion The As2O3 can obviously inhibit the proliferation of the human epidermal carcinoma cells cultured in vitro,and can induce the cells' apoptosis,which may be related to its inhibition effect on the expression of Survivin.