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2011•Junshi yixueRequires access

Influence of Spred2 on apoptosis of human hepatocellular carcinoma cells

Xiao-Ni Ma

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Abstract

Objective To observe the effect of Spred2 on apoptosis of human hepatocellular carcinoma cells. Methods HepG2 cells were transfected with pcDNA3. 0 and pcDNA3. 0-Spred2 for the morphologic change of HepG2 to be observed. Cells were stained with annexin Ⅴ conjugated to fluorescein isothiocyanate(annexin Ⅴ-FITC) and propidium iodide(PI/7-AAD) . The stained cells were analyzed on a flow cytometer using the Cellquest program. The expression level of Spred2 was examined by Western blot. Results Spred2 over-expression induced HepG2 cells apoptosis and sensitized cells to 5-FU induced HepG2 cells apoptosis. Conclusions Spred2 can regulate HepG2 cellular apoptosis.

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Objective To observe the effect of Spred2 on apoptosis of human hepatocellular carcinoma cells. Methods HepG2 cells were transfected with pcDNA3. 0 and pcDNA3. 0-Spred2 for the morphologic change of HepG2 to be observed. Cells were stained with annexin Ⅴ conjugated to fluorescein isothiocyanate(annexin Ⅴ-FITC) and propidium iodide(PI/7-AAD) . The stained cells were analyzed on a flow cytometer using the Cellquest program. The expression level of Spred2 was examined by Western blot. Results Spred2 over-expression induced HepG2 cells apoptosis and sensitized cells to 5-FU induced HepG2 cells apoptosis. Conclusions Spred2 can regulate HepG2 cellular apoptosis.

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Available abstract

Objective To observe the effect of Spred2 on apoptosis of human hepatocellular carcinoma cells. Methods HepG2 cells were transfected with pcDNA3. 0 and pcDNA3. 0-Spred2 for the morphologic change of HepG2 to be observed. Cells were stained with annexin Ⅴ conjugated to fluorescein isothiocyanate(annexin Ⅴ-FITC) and propidium iodide(PI/7-AAD) . The stained cells were analyzed on a flow cytometer using the Cellquest program. The expression level of Spred2 was examined by Western blot. Results Spred2 over-expression induced HepG2 cells apoptosis and sensitized cells to 5-FU induced HepG2 cells apoptosis. Conclusions Spred2 can regulate HepG2 cellular apoptosis.

Key concepts: Propidium iodide, Apoptosis, Annexin, Fluorescein isothiocyanate, Transfection, Hepatocellular carcinoma, Flow cytometry, Isothiocyanate

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