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2008•Chinese Journal of Experimental and Clinical Infectious DiseasesRequires access

Detection of apoptosis of the human hepatic carcinoma cells by Annexin V/PI double staining methods

Meng Qing-hu

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Abstract

Objective To investigate the property of apoptosis of HepG2 cell line induced by CDDP.Methods Liver cancer cell line HepG2 was incubated with CDDP of different concentrations for 4 h,15 h,24 h,36 h,respectively.Apoptosis was measured by flow cytometry method and Annexin V and PI double staining.Results Apoptosis of HepG2 cell could be induced by CDDP,the percentage of AnnexinⅤ+ PI cells increased along with the increase of CDDP concentration and incubation time.Conclusions Apoptosis of HepG2 cell induced by CDDP was time and dose dependent.Annexin V and PI double staining could be a specific method for analyzing apoptotic cells.

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What this paper is about

Objective To investigate the property of apoptosis of HepG2 cell line induced by CDDP.Methods Liver cancer cell line HepG2 was incubated with CDDP of different concentrations for 4 h,15 h,24 h,36 h,respectively.Apoptosis was measured by flow cytometry method and Annexin V and PI double staining.Results Apoptosis of HepG2 cell could be induced by CDDP,the percentage of AnnexinⅤ+ PI cells increased along with the increase of CDDP concentration and incubation time.Conclusions Apoptosis of HepG2 cell induced by CDDP was time and dose dependent.Annexin V and PI double staining could be a specific method for analyzing apoptotic cells.

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Available abstract

Objective To investigate the property of apoptosis of HepG2 cell line induced by CDDP.Methods Liver cancer cell line HepG2 was incubated with CDDP of different concentrations for 4 h,15 h,24 h,36 h,respectively.Apoptosis was measured by flow cytometry method and Annexin V and PI double staining.Results Apoptosis of HepG2 cell could be induced by CDDP,the percentage of AnnexinⅤ+ PI cells increased along with the increase of CDDP concentration and incubation time.Conclusions Apoptosis of HepG2 cell induced by CDDP was time and dose dependent.Annexin V and PI double staining could be a specific method for analyzing apoptotic cells.

Key concepts: Annexin, Apoptosis, Pi, Staining, Flow cytometry, Molecular biology, Cell culture, Annexin A5

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