Protective Effect of 7-difluoromethylgenistein on Injury Induced by Hydrogen Peroxide in PC12 Cell Line

Objective To investigate the protective effect of 7-difluoromethylgenistein on the damage of PC12 cells induced by oxidative stress.Methods H2O2-induced PC12 cell damage was employed as the model of neuron damage induced by oxidative stress.the proliferation of PC12 cells was determined by 3-[4,5-dimethylthiazol-2-yl ]-2,5-diphenyltetrazolium bromide(MTT) assay,the apoptosis of PC12 cells was detected by flow cytometry(FCM) with propidium iodide stain,the LDH activity of PC12 cells function by multifunction biochemistry analysator.Results In the presence of 7-difluoromethylgenistein,both the inhibitory rates of PC12 cells and the LDH release of PC12 cells decreased.,and the apoptosis of PC12 cells decreased as well after 40μmol/L H2O2 for 24 h.Conclusion 7-difluoromethylgenistein has protective effect on the damage of PC12 cells induced by oxidative stress.