Protective Effect of Hydrogen Sulfide on H_2O_2-Induced Damage in PC12 Cells

Objective To study the protective effect of hydrogen sulfide (H2S) on PC12 cells damage induced by oxidative stress.Methods Using H2O2-induced PC12 cells damage as the model of neuron damage induced by oxidative stress,the cell shape and nuclear morphology were observed by nuclear staining with hoechst 33258,the proliferation of PC12 cells was observed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay,the apoptosis of PC12 cells was detected by propidium iodide stain flow cytometry(FCM).Results Exposure to H2O2,the apoptosis of PC12 cells preconditioned by H2S as measured by hoechst stain was significantly decreased,compared with no-preconditioned cells.In the present of H2S(200 and 400 μmol/L),the inhibitory rates of PC12 cells induced by H2O2 from 200 to 400 μmol/L and the apoptosis of PC12 cell induced by 200 and 400 μmol/L H2O2 for 24 h were decreased.Conclusions H2S has the protective effect on PC12 cells damage induced by oxidative stress.