Anti-fungi activities and cultural characteristics of ginkgo endophytic fungus No.1028

Endophytic fungus No.1028,was identified as Nigrospora sp. based on morphological study and sequence analysis of rDNA-ITS, isolated from the stem of Gingko biloba L. by aseptic techniques. The metabolites of this endophytic fungus were found to have strong inhibition effect on several crop pathogenic fungi in terms of inhibition rate in hypha growth. The inhibition rates were 66.7%, 48.3%, 64.6%, 36.5%, 57.1%, 23.0% on pathogenic fungi Alternaria solani, Fusarium oxysporum, Colletotrichum lindemuthianum, Colletotrichum gloeosporioides, Thanatephorus cucumeris and Rhizoctonia solani, respectively. The active ingredient was separated and purified out from fermentation liquid of endophytic fungus No.1028. It was white crystalline powder by extraction. The effects of carbon, nitrogen and inorganic ion were studied on the growth and metabolism of this endophytic fungus. Corn starch, soybean starch and Na+ could obviously promote its growth and metabolism for significantly producing more active ingredients.