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Identification of t(12;21) in Chinese Pediatric Cases with Acute Lymphoblastic Leukemia by Interphase Fluorescence in Situ Hybridization

Liu Shi-he

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Abstract

Objective To discuss the role of interphase flu orescence in situ hybridization(I-FISH) in t(12;21) detection in Chinese pediat ric cases with acute lymphoblastic leukemia.Methods t(12; 21)/TEL-AML1 fusion gene was identified in bone marrow or peripheral blood mono nuclear cells from 51 newly diagnosed childhood ALL patients by dual color I-F ISH and conventional cytogenetic analysis(CCA).Results 11 cases were found with a t(12;21) by FISH.The incidence of the t(12;21) was 21. 6% in newly diagnosed pediatric ALLs.Only one case had a dubious t(12;21) when d etecting by CCA.Conclusion t(12;21) is the most common cy togenetic translocations in Chinese pediatric ALLs,but it always cannot be ident ified by routine CCA.Other molecular methods,e.g.FISH are powerful in detecting such a cryptic genetic translocation.

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Objective To discuss the role of interphase flu orescence in situ hybridization(I-FISH) in t(12;21) detection in Chinese pediat ric cases with acute lymphoblastic leukemia.Methods t(12; 21)/TEL-AML1 fusion gene was identified in bone marrow or peripheral blood mono nuclear cells from 51 newly diagnosed childhood ALL patients by dual color I-F ISH and conventional cytogenetic analysis(CCA).Results 11 cases were found with a t(12;21) by FISH.The incidence of the t(12;21) was 21. 6% in newly diagnosed pediatric ALLs.Only one case had a dubious t(12;21) when d etecting by CCA.Conclusion t(12;21) is the most common cy togenetic translocations in Chinese pediatric ALLs,but it always cannot be ident ified by routine CCA.Other molecular methods,e.g.FISH are powerful in detecting such a cryptic genetic translocation.

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Available abstract

Objective To discuss the role of interphase flu orescence in situ hybridization(I-FISH) in t(12;21) detection in Chinese pediat ric cases with acute lymphoblastic leukemia.Methods t(12; 21)/TEL-AML1 fusion gene was identified in bone marrow or peripheral blood mono nuclear cells from 51 newly diagnosed childhood ALL patients by dual color I-F ISH and conventional cytogenetic analysis(CCA).Results 11 cases were found with a t(12;21) by FISH.The incidence of the t(12;21) was 21. 6% in newly diagnosed pediatric ALLs.Only one case had a dubious t(12;21) when d etecting by CCA.Conclusion t(12;21) is the most common cy togenetic translocations in Chinese pediatric ALLs,but it always cannot be ident ified by routine CCA.Other molecular methods,e.g.FISH are powerful in detecting such a cryptic genetic translocation.

Key concepts: Fluorescence in situ hybridization, Chromosomal translocation, Interphase, Fish <Actinopterygii>, Lymphoblastic Leukemia, Bone marrow, In situ hybridization, Biology

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Identification of t(12;21) in Chinese Pediatric Cases with Acute Lymphoblastic Leukemia by Interphase Fluorescence in Situ Hybridization — Research Paper | ScholarLens