Value of Interphase Fluorescence in Situ Hybridization in Identification of TEL-AML1 Fusion Gene in Pediatric Cases with Acute Lymphoblastic Leukemia

Objective To discuss the role of interphase fluorescence in situ hybridization(I-FISH) in(t12;21) detection in pediatric cases with acute lymphoblastic leukemia.Methods TEL-AML1 fusion gene was identified in bone marrow mononuclear cells from 31 newly diagnosed childhood ALL patients by dual color I-FISH and conventional cytogenetic analysis(CCA).Results 7 cases were found with a TEL-AML1 fusion gene by FISH.The incidence of the(t12;21) was 22.6% in newly diagnosed pediatric ALLs.None of those cases had a dubious TEL-AML1 fusion gene when detecting by CCA.Conclusion Dual color I-FISH is more sensitive and characteristic than conventional cytogenetic analysis(CCA) in the identification of TEL-AML1 fusion gene.So it is palying an significant role in diagnosis and therapy of pediatric cases with acute lymphoblastic leukemia.