Effects of the ambroxol on E-selectin and intercellular adhesion molecule-1 in acute lung injury of rabbits

OBJECTIVE To investigate the changes of E-selectin,intercellular adhesion molecule-1 of lung tissue during acute lung injury(ALI)induced by oleic acid.To determine the protection effects of ambroxol injection on acute lung injury and its mechanisms.METHODS The acute lung injury model of rabbit was induced by oleic acid.Twenty-four Japanese white rabbit were divided into three groups randomLy:normal saline group(NS group),acute lung injury group(OA group),acute lung injury plus ambroxol injection group(AMB group).As the model of ALI was induced successfully,the rabbits in OA group and NS received normal saline,and in AMB group received ambroxol injection through auricular vein.The rabbits were sacrificed after 6 hours.The content of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),E-selectin,intercellular adhesion molecule-1(ICAM-1)in both bronchoalveolar lavage fluid(BALF)and lung tissue homogenate were measured.The wet dry ratio(W/D)of lung tissue were tested.The pathological alternation in lung tissue were observed under microscope.RESULTS The W/D of lung tissue in AMB group was significant decreased compared with ALI group(P0.01),but was significant higher than NS group(P0.05).Alveolitis were decreased compared with ALI group.The content of TNF-α,IL-1β,E-selectin and ICAM-1 both in BALF and in lung tissue homogenate in AMB group decreased significantly compared with ALI group(P0.01),but were higher than NS group(P0.01).The content of E-selectin,ICAM-1 correlated intensively with IL-1β,TNF-α in BALF or lung tissue homogenate respectively.CONCLUSIONS Ambroxol has protection effect on oleic acid-induced acute lung injury.Ambroxol could inhibit the release of proinflammatory cytokines(ie IL-1β and TNF-α)and inhibit the expression of cellular adhesion molecules(ie E-selectin and ICAM-1),prevent the polymorphonuclear leukocytes from adhering endothelial cells and migratting to sites of inflammation for protecting the lung against acute lung injury.