STUDY ON INDUCTION AND PROPAGATION OF CALLUS IN TISSUE CULTURE OF COMMON BUCKWHEAT

In this paper,it were studied that effect of H 2O 2 and HgCl 2 solution to sterilization of common buckwheat seeds and phytohormone affect to induction and propagation of callus. Contamination rate were from 0 to 6.2% and germination rate were above 92% after seeds of 5 varieties (Yu3-3,Limadao,Pujiang,Longshan and Fuyuanhonghua) were sterilized with 10% H 2O 2 solution for 150min.It was applicable to seeds of 3 varieties (8621,B-Dali and America) to sterilize with 0.1% HgCl 2 solution for 12min, contamination rate of seeds were 0 and germination rate were from 56.1% to 68.7%. On media supplemented with 0.5 ～4.0mg/L 2,4-D,cotyledon and hypocotyl explants from sterilized seedlings were dedifferentiated and calli were formed. Use of BA was very importent in medium containing 2,4-D ,it was helpful for propagation of callus and was unfavourable for differentiation of roots.