Establishment of Callus-induction and in vitro Regeneration System in Buckwheat

In this paper,cotyledons and hypocotyls obtained from aseptic seedling of buckwheat variety Beizaosheng were used as explants to research the culture conditions of buckwheat callus induction and callus differentiation,in order to establish the buckwheat tissue culture regeneration system.The results showed that the artificial peeled seeds cultivated in light incubator with the conditions of 1/2MS medium and 25 ℃ constant temperature could grow into a large number of robust aseptic seedlings;in the MS medium added with 2 mg/L 2,4-D and 1 mg/L 6-BA,induction rate of hypocotyl callus were 100% and the induction rate of cotyledon were more than 90%;in the MS medium added with 2 mg/L 6-BA and 1.5 mg/L KT,differentiation rate of cotyledon-derived callus were(64.0±6.9)%,and in the MS medium added with 2 mg/L 6-BA and 0.5 mg/L KT,differentiation rate of hypocotyl-derived callus were(47.5±4.8)%;in the1/2 MS medium added with 0.6 mg/L NAA,the rooting rate of adventitious shoots were 100%,and the plant survival rate could be more than 70% after seedlings hardening.