Relationship between the polymorphism of TNFα-308G/A,TNFβG252A and Alzheimer’s disease

Objective To investigate the relationship between plasma levels of tumor necrosis factor(TNF) or its polymorphism and Alzheimer’s disease.Methods Plasma level of TNF was measured by RIA and the polymorphism of TNFα-308G/A and TNFβG252A was screened by PCR-RELP.Results The gene frequency of TNFα-308G/A in AD group was as following: and TNFα-308G/A 1/1,1/2 and 2/2 genotype frequency: 0.8636,0.1212 and 0.0152, TNFα-308G/A 1 and TNFα-308G/A 2 Genefrequency: 0.9242 and 0.0758。the gene frequency of TNFα-308G/A in control group was as followed; TNFα-308G/A 1/1、1/2、2/2 genotype frequency: 0.8881,0.1049 and 0.070, TNFα-308G/A 1、TNFα-308G/A 2 Genefrequency: 0.9021 and 0.0979,The gene frequency of TNFβG252A in AD group was as following; TNFβG252A 1/1,1/2 and 2/2 genotype frequency: 0.242, 0.591 and 0.167, TNFβG252A 1 and TNFβG252A 2 gene frequency: 0.252,0.573 and 0.175. The gene frequency of TNFβG252A in control group was as following; TNFβG252A 1/1,1/2 and 2/2 genotype frequency: 0.252,0.573 and 0.175, TNFβG252A 1 and TNFβG252A 2 Genefrequency: 0.9021 and 0.0979.There were no significant differences in the frequencies of TNFα-308G/A and TNFβG252A gene between the patients and control group( P0.05).Alzheimer’s disease group showed significantly higher plasma level of TNF than the control group(P0.05).Conclusion Plasma TNF increases in Alzheimer’s disease. The results suggested: TNF may play important role in the procession of AD and no evident association was found between TNFα-308G/A or TNFβG252A polymorphism and Alzheimer’s disease.