HPLC Determination of Ellagic Acid and Gallic Acid in Fermented Blueberry

Two methods of High-performance Liquid Chromatography were established to determinate the ellagic acid content and the gallic acid content in fermented blueberry.The measurement conditions of ellagic acid were Waters symmetry C18column(250 × 4.6mm,5μm),column temperature25℃,the mobile phase 46∶2∶52 methanol-ethyl acetate-KH2PO4/H3PO4 solution,flow rate 0.8 m L/min,and detection wavelength 254 nm,the average detection recovery rate of ellagic acid in blueberries fermentation was 92.68%,RSD was0.97%.The gallic acid measurement conditions of were Waters symmetry C18column(250 × 4.6mm,5μm),the column temperature35℃,the mobile phase was methanol:water(0.1% phosphoric acid) 10∶90,flow rate 1.0 m L/min,detection wavelength 273 nm,the average detection recovery rate of gallic acid in blueberries fermentation was 99.0%,RSD was 0.73%.Both the methods were simple,fast and reproclucible,and could determine the content of ellagic acid and gllic acid in fermented blueberry accurately.