ScholarLens Back to search
2012•Food Science and Technology InternationalRequires access

HPLC determination of gallic acid in cane sugar juice

Jinmei Lin

Open publisher page 2 citations

Abstract

Aim To establish a method for the detection of gallic acid in cane sugar juice.Methods:HPLC was on a Symmetry C18 analytical colume(4.6 mm×250 mm,5 μm) with acetonitrile—0.5% phosphoric acid and flow rate was 1.0 mL/min.The UV detection wavelength was set at 280nm and column temperature was 25 ℃.Results:The linear range of gallic acid was 20～100 mg/L,the regression equation was Y=15960X-4749(r=0.9991) ,with a detection limit of 0.22 mg/L The recover of measure in analyzing of gallic was 101.45%(RSD=1.13%) .The clarified sugar juice and syrup were prepared into the concentration of 15 °Bx,and then filtrated by 0.45 micro-filtration membrane,the content of gallic acid were 21.70 mg/L,17.06 mg/L respectively.Conclusions:The HPLC method was accurate and reliable in analyzing the gallic acid of cane sugar juice.

About this research paper

What this paper is about

Aim To establish a method for the detection of gallic acid in cane sugar juice.Methods:HPLC was on a Symmetry C18 analytical colume(4.6 mm×250 mm,5 μm) with acetonitrile—0.5% phosphoric acid and flow rate was 1.0 mL/min.The UV detection wavelength was set at 280nm and column temperature was 25 ℃.Results:The linear range of gallic acid was 20～100 mg/L,the regression equation was Y=15960X-4749(r=0.9991) ,with a detection limit of 0.22 mg/L The recover of measure in analyzing of gallic was 101.45%(RSD=1.13%) .The clarified sugar juice and syrup were prepared into the concentration of 15 °Bx,and then filtrated by 0.45 micro-filtration membrane,the content of gallic acid were 21.70 mg/L,17.06 mg/L respectively.Conclusions:The HPLC method was accurate and reliable in analyzing the gallic acid of cane sugar juice.

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Aim To establish a method for the detection of gallic acid in cane sugar juice.Methods:HPLC was on a Symmetry C18 analytical colume(4.6 mm×250 mm,5 μm) with acetonitrile—0.5% phosphoric acid and flow rate was 1.0 mL/min.The UV detection wavelength was set at 280nm and column temperature was 25 ℃.Results:The linear range of gallic acid was 20～100 mg/L,the regression equation was Y=15960X-4749(r=0.9991) ,with a detection limit of 0.22 mg/L The recover of measure in analyzing of gallic was 101.45%(RSD=1.13%) .The clarified sugar juice and syrup were prepared into the concentration of 15 °Bx,and then filtrated by 0.45 micro-filtration membrane,the content of gallic acid were 21.70 mg/L,17.06 mg/L respectively.Conclusions:The HPLC method was accurate and reliable in analyzing the gallic acid of cane sugar juice.

Key concepts: Gallic acid, Chemistry, Chromatography, High-performance liquid chromatography, Phosphoric acid, Sugar, Detection limit, Linear range

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
HPLC determination of gallic acid in cane sugar juice — Research Paper | ScholarLens