2010•Journal of Youjiang Medical University for NationalitiesRequires access
The killing action of Astragalus Polysaccharides in combination with paclitaxel on tumor cells
Wei Peng-ya
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Abstract
Objective To study the effects of Astragalus Polysaccharides on MCF-7 cell line cycle of mammary cancer and its killing action on MCF-7 cell when in combination with paclitaxel.Methods A methylthiazolyltetrazolium(MTT) test was performed to measure the inhibition rate of cell proliferation so as to evaluate the inhibition of Astragalus Polysaccharides on MCF-7 cell proliferation.A flow cytometer was used to test whether the cell cycles had changed or not and to measure the apoptosis rate. Results Astragalus Polysaccharides can inhibit the growth and proliferation of MCF-7 cells.The killing action of Astragalus Polysaccharides in combination with paclitaxel on MCF-7 cell is superior to Astragalus Polysaccharides alone or paclitaxel alone.After treatment with Astragalus Polysaccharides,hypodiploid apoptosis occurred which DNA content of MCF-7 cells was lower than that in stage G1,the cells cycle was blocked in stage G1.Conclusion Astragalus Polysaccharides can kill MCF-7 cells and its combination with paclitaxel can have synergic anti-neoplastic function.