Expression of interleukin-10 around the hematoma and its correlation with cerebral edema in rats with intracerebral hemorrhage

Objective To explore the expression of interleukin-10 (IL-10)around the hematoma and its correlation with cerebral edema in rats with intracerebral hemorrhage(ICH). Methods 130 SD rats were randomly divided into normal control group,sham-operated group and ICH group. The ICH models were established by heparinized Ⅶ-type collagenase. Then at ICH 12 h,24 h,48 h,72 h,7 d and 14 d,the scores of neurological deficit were evaluated by Bederson scale. The cerebral water content was checked up by the dry / wet weight method. And the expression of IL-10 around the hematoma in cerebral tissues was detected by immunohistochemistry.Results (1)In ICH group,the neurological deficit scores and cerebral water content were step up at ICH 12 h,reAChEd the maximum at 48 h,and went back to normal at 7 d. (2) The expression of IL-10 in cerebral tissues in ICH group was obviously increased at ICH 12 h,and reached the peak at 14 d,and was significantly higher than which in normal control group and sham-operated group at each time point(P0.05-0.01). (3)In ICH group,neurological deficit score was positive correlated with cerebral water content (r=0.761,P0.01).The expression of IL-10 was negative associated with the cerebral water content and neurological deficit scores (r=-0.65,-0.753,all P0.01).Conclusions IL-10 around the hematoma has the tendency of increasing gradually in the ICH rats,and it may take part in the protective effects of reducing brain edema and improve neurological recovery after ICH.