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2002•Zhonghua weishengwuxue he mianyixue zazhiRequires access

Genetic characterization of four stains Borrelia burgdorferi isolated in China

Xia Zeng, Shusheng Wang, Yi Min Liu, Tao Zhang, Shengli Bi, Yongdong Zhou

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Abstract

Objective Genetic characterization of four strains of Borrelia burgdorferi isolated in China. Methods PCR technique was used to amplify the 5s 23s rRNA intergenic spacer DNA from the whole cellular DNA of isolates GXLD 4,9,18 and Chang 14. The amplified products were then cloned into plasmid pGEM T Easy and sequenced. Results The 5s 23s rRNA/intergenic spacer DNA of the four isolates was 242bp, revealing a nucleotide sequence identity of 99%. The four isolates had higher sequence identified with B.valaisiana than with other genomic groups. Conclusion The four isolates most likely belong to Borrelia valaisiana genomic group.

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What this paper is about

Objective Genetic characterization of four strains of Borrelia burgdorferi isolated in China. Methods PCR technique was used to amplify the 5s 23s rRNA intergenic spacer DNA from the whole cellular DNA of isolates GXLD 4,9,18 and Chang 14. The amplified products were then cloned into plasmid pGEM T Easy and sequenced. Results The 5s 23s rRNA/intergenic spacer DNA of the four isolates was 242bp, revealing a nucleotide sequence identity of 99%. The four isolates had higher sequence identified with B.valaisiana than with other genomic groups. Conclusion The four isolates most likely belong to Borrelia valaisiana genomic group.

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Available abstract

Objective Genetic characterization of four strains of Borrelia burgdorferi isolated in China. Methods PCR technique was used to amplify the 5s 23s rRNA intergenic spacer DNA from the whole cellular DNA of isolates GXLD 4,9,18 and Chang 14. The amplified products were then cloned into plasmid pGEM T Easy and sequenced. Results The 5s 23s rRNA/intergenic spacer DNA of the four isolates was 242bp, revealing a nucleotide sequence identity of 99%. The four isolates had higher sequence identified with B.valaisiana than with other genomic groups. Conclusion The four isolates most likely belong to Borrelia valaisiana genomic group.

Key concepts: Borrelia burgdorferi, Biology, 23S ribosomal RNA, genomic DNA, Intergenic region, Genetics, Ribosomal RNA, DNA

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