Three methods for detecting the apoptosis of PAJU cells induced by amyloid beta-protein

Objective To explore application of MTT,Hoechst 33258 staining and flow cytometry detecting the apoptosis of PAJU cells induced by amyloid beta-protein under hypoxia condition.Methods PAJU cells were divided into ①normal group: 50 ml/L culturing PAJU cells in CO2 box for 96 h;②Aβ group:giving PAJU cells 1.0 nmol/L aged Aβ42,then culturing them for 96 h;③hypoxia group: culturing PAJU cells in 1%～2% O2,5% CO2,93% N2 box for 96 h;④Aβ+hypoxia group: giving PAJU cells 1.0 nmol/L aged Aβ42,culturing them in 1%～2% O2,5% CO2,93% N2 box for 96 h.Apoptosis of PAJU cells were observed by MTT,Hoechst 33258 staining and flow cytometry methods respectively.Results The number of PAJU cells of Aβ,hypoxia and Aβ+ hypoxia groups were decreased,and the activity of them were decreased compared with controlled ones in MTT,Hoechst 33258 staining and flow cytometry respectively.There was statistically significance between control and experimental groups(P0.05),and there was no statistically significance between Aβ group and hypoxia groups in apoptosis rate(P0.05).Conclusions Aβ42 could cause the apoptosis of PAJU cells,hypoxia could enhance the apoptosis.