2011•Gansu Agricultural Science and TechnologyRequires access

Study on Culture of in Vitro Rooting of Test-tube Plantlet of Almond's Rootstocks of Nemaguard and Lovell

Yuliang Chen

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Abstract

The Rooting of Almond's rootstocks of Nemaguard and Lovel1 had been researched in this paper.The results showed that the rooting medium of 1/2 MS+0.5 mg/L IBA was best medium,which had the highest rooting rate of test-tube plantlet.The dark treatment in the early rooting could improve the rooting rate,it was best to use the medium of 1/2 MS+0.5 mg/L IBA to induce root and culture in dark for 10 days.Added 1g/L activated charcoal(AC) could improve rooting rate,but when the AC concentrations continue to add,the rooting rate was decline.The rooting effect on socking of the base of the shoots is obvious by 100 mg/L IBA solution,however,the root was thin and less.The shoots was first cultured in the medium of 1/2 MS +1.0 mg/L IBA for 6 days,then transplanted into the medium of 1/2 MS,which could promote rooting.

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What this paper is about

The Rooting of Almond's rootstocks of Nemaguard and Lovel1 had been researched in this paper.The results showed that the rooting medium of 1/2 MS+0.5 mg/L IBA was best medium,which had the highest rooting rate of test-tube plantlet.The dark treatment in the early rooting could improve the rooting rate,it was best to use the medium of 1/2 MS+0.5 mg/L IBA to induce root and culture in dark for 10 days.Added 1g/L activated charcoal(AC) could improve rooting rate,but when the AC concentrations continue to add,the rooting rate was decline.The rooting effect on socking of the base of the shoots is obvious by 100 mg/L IBA solution,however,the root was thin and less.The shoots was first cultured in the medium of 1/2 MS +1.0 mg/L IBA for 6 days,then transplanted into the medium of 1/2 MS,which could promote rooting.

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Available abstract

The Rooting of Almond's rootstocks of Nemaguard and Lovel1 had been researched in this paper.The results showed that the rooting medium of 1/2 MS+0.5 mg/L IBA was best medium,which had the highest rooting rate of test-tube plantlet.The dark treatment in the early rooting could improve the rooting rate,it was best to use the medium of 1/2 MS+0.5 mg/L IBA to induce root and culture in dark for 10 days.Added 1g/L activated charcoal(AC) could improve rooting rate,but when the AC concentrations continue to add,the rooting rate was decline.The rooting effect on socking of the base of the shoots is obvious by 100 mg/L IBA solution,however,the root was thin and less.The shoots was first cultured in the medium of 1/2 MS +1.0 mg/L IBA for 6 days,then transplanted into the medium of 1/2 MS,which could promote rooting.

Key concepts: Plantlet, Rootstock, Shoot, Horticulture, Biology, Botany, Tissue culture, In vitro

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