Analysis on comparative biology of hairless gene in Yuyi hairless mice

Aim: To study the difference of hairless gene between Yuyi hairless mice and others species.Methods:RT-PCR technique was developed to clone and analyze the sequence of cDNA in hairless gene from Yuyi hairless mice,and to analyze comparatively biologic character of amino acid sequence encoding hairless gene in different species.Results: A full-length cDNA of Yuyi hairless mice was 4 014 bp,which has been accepted by GenBank(Accession Number: AY547390).The identities of nucleotide of hairless gene were 99.9%,99.7%,94.3%,81.7%,81.8% by homologous comparison among Yuyi hairless mice and other species,and those of amino acid sequences were 99.8%,99.7%,94.5%,79.8%,80.0%,respectively.Conclusion: The results suggest that the hairless gene is a highly-conserved gene and has higher homology among different mammalian taxons.