Determination of Three Stilbenes Residues in Products of Animals by UPLC-MS/MS

A method based on ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) has been developed for the determination of stilbenes residues including diethylstilbestrol,hexestrol and dienoestrol in products of animal origin.The homogenized samples were hydrolysised with beta-glucuronidase and the analytes were extracted with tert-butyl methyl ether.Then the extract was cleaned by silica solid phase extraction cartridge.Quantification of the three analytes was achieved by UPLC-MS/MS with multiple reaction monitoring(MRM) using internal standard method.Detection limits and quantification limits of UPLC-MS/MS for the three analytes were all 0.1 μg/kg and 0.5 μg/kg.The overall recovery varies from 75.8%~110.0% at the spiked level of 1.0,1.5,2.0 μg/kg,and the relative standard deviation(RSD) of the method was from 3.3%~13.2%.The method was reliable,sensitive and reproducibility,its performance can meet the requirements of the domestic and international legislation.The method adapts to the determination of stilbenes residues in different products of animal origin.