Determination of Four Beta-Agonists Residues in Products of Animal Origin and Urine by Ultra Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

A method based on ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) has been developed for the determination of beta-agonists residues including clenbuterol,ractopamine,salbutamol and terbutalin in products of animal origin and unine.The analytes were extracted with NH4Ac solution from homogenized samples and hydrolysised with beta-glucuronidase overnight at 37 ℃.Then the extract was filtered and the filtrate was cleaned by Oasis MCX solid phase extraction cartridge.Quantification of the four analytes was achieved by UPLC-MS/MS with multiple reaction monitoring(MRM) using internal standard method.Detection limits and quantification limits of UPLC-MS/MS for the four analytes were all 0.1 mg/kg and 0.5 mg/kg.The overall recovery varies from 86.7% to 114.3% at the spiked level of 0.5 mg/kg,0.75 mg/kg,1.0 mg/kg,and the relative standard deviation(RSD) of the method was less than 10%.The method is reliable,sensitive and reproducible,its performance can meet the requirements of the domestic and international legislation.The method adapts to the determination of beta-agonists residues in different products of animal origin and urine.