Analysis on genetic diversity of Atractylodes lancea populations based on RAPD marker

By means of RAPD marker,PCR amplification of genomic total DNA of 28 individuals in seven wild populations of Atractylodes lancea(Thunb.) DC.distributing in Xiaojiuhuashan,Xiaotangshan and Hushan of Jiangsu Province,Jinzhai and Wuhu of Anhui Province and Baokang and Yingshan of Hubei Province was carried out.On this basis,genetic diversity and genetic differentiation of these populations were analyzed,and their genetic relationship was also analyzed by cluster analysis method.The results show that 193 bands are amplified by 18 RAPD primers,in which there are 111 polymorphic bands with a polymorphic band percentage(PPB) of 57.51%.Average band number amplified by one primer is 10.72 bands,in which there are 6.17 polymorphic bands.On provincial level,PPB,effective alleles number(Ne),Nei's gene diversity index(H) and Shannon's information index(I) of populations of Anhui Province all are the lowest,while Ne,H and I of populations of Hubei Province all are the highest,but PPB of populations of Jiangsu Province is the highest.On population level,PPB,Ne,H and I of Baokang population of Hubei Province all are the highest,while those of Jinzhai population of Anhui Province all are the lowest.Gene differentiation coefficient and gene flow of seven populations is 0.206 5 and 1.912 5,respectively,indicating that 20.65% of overall genetic variation of seven populations exists among populations and 79.35% within populations.Genetic distance among seven populations is 0.150 7-0.252 1,in which,that between populations of Jinzhai and Wuhu of Anhui Province isthesmallest(0.150 7) and thatbetween populations ofHushan of JiangsuProvince and Wuhu of Anhui Province is the biggest(0.252 1).Cluster analysis result according to genetic distance shows that seven populations can be divided into two groups,only population of Baokang of Hubei is alone in a group and the other six populations are clustered in another group.And all of individuals from the same population can be clustered together.It is suggested that genetic diversity among A.lancea populations is lower and there is no obvious genetic differentiation among populations.