Determination of Selenium and Other Heavy Metals in Human Urine by ICP-MS with Microwave Digestion

The method for the determination of selenium and other heavy metals,such as chromium,manganese,cobalt,nickel,cadmium,lead,arsenic,copper and zinc,in human urine was established by inductively coupled plasma-mass spectrometry(ICP-MS) with microwave digestion. The samples were prepared by microwave digestion with HNo_3-H_2O_2 digestion system,and the 10 kind of elements were directly determined by ICP-MS with indium and bismuth as internal standard substance.The detection limits of the 10 elements were in the range of 0.006—0.073μg/L,and the standard curve had the good linear relationship with correlation coefficient above 0.9999 and RSD less than 3%,and the measured value of urine standard substance were all in the standard reference ranges.The urine samples were analyzed by ICP-MS with microwave digestion,and the method is simple and fast with high sensitivity,low detection limit and good repeatability,that is an ideal method for analysis and detection of biological samples.