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Expression and Electrophysioloyical Record of Human HCN2 Gene

Xiangjun Yang

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Abstract

Objective To identify successful transfection of hHCN2 gene into HEK293 cells by liposome method and create a model for studying ionic channels.Methods hHCN2 was transfected with lipofectamine 2000 into HEK293 line cells.Whole-cell patch clamp indicated that hHCN2 gene was transfected into HEK293 cells.Results Whole-cell patch clamp recorded ionic currents of transfected hHCN2.Conclusion The techniques we used are dependable and will be helpful for us study the relationship between the structure and function of cloned ionic channels.

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What this paper is about

Objective To identify successful transfection of hHCN2 gene into HEK293 cells by liposome method and create a model for studying ionic channels.Methods hHCN2 was transfected with lipofectamine 2000 into HEK293 line cells.Whole-cell patch clamp indicated that hHCN2 gene was transfected into HEK293 cells.Results Whole-cell patch clamp recorded ionic currents of transfected hHCN2.Conclusion The techniques we used are dependable and will be helpful for us study the relationship between the structure and function of cloned ionic channels.

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Available abstract

Objective To identify successful transfection of hHCN2 gene into HEK293 cells by liposome method and create a model for studying ionic channels.Methods hHCN2 was transfected with lipofectamine 2000 into HEK293 line cells.Whole-cell patch clamp indicated that hHCN2 gene was transfected into HEK293 cells.Results Whole-cell patch clamp recorded ionic currents of transfected hHCN2.Conclusion The techniques we used are dependable and will be helpful for us study the relationship between the structure and function of cloned ionic channels.

Key concepts: Lipofectamine, Transfection, HEK 293 cells, Patch clamp, Cell culture, Gene, Molecular biology, Cell biology

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