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2006•Jiangsu Medical JournalRequires access

Transfection of human HCN2 gene into HEK293 cells by liposome method

Xia Bai

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Abstract

Objective To create a model for studying ionic channels. Methods pcDNA3- hHCN2 was transfected with lipofectamine 2000 into HEK293 Cells.The expression of hHCN2 gene was detected by whole-cell patch clamp. Results Whole-cell patch clamp recorded ionic currents of HEK293 cells after transfected pcDNA3-hHCN2 three days later. Conclusion The technique is reliable and consistent, which will be helpful for studying the relationship between the structure and function of cloned ionic channels.

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What this paper is about

Objective To create a model for studying ionic channels. Methods pcDNA3- hHCN2 was transfected with lipofectamine 2000 into HEK293 Cells.The expression of hHCN2 gene was detected by whole-cell patch clamp. Results Whole-cell patch clamp recorded ionic currents of HEK293 cells after transfected pcDNA3-hHCN2 three days later. Conclusion The technique is reliable and consistent, which will be helpful for studying the relationship between the structure and function of cloned ionic channels.

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Available abstract

Objective To create a model for studying ionic channels. Methods pcDNA3- hHCN2 was transfected with lipofectamine 2000 into HEK293 Cells.The expression of hHCN2 gene was detected by whole-cell patch clamp. Results Whole-cell patch clamp recorded ionic currents of HEK293 cells after transfected pcDNA3-hHCN2 three days later. Conclusion The technique is reliable and consistent, which will be helpful for studying the relationship between the structure and function of cloned ionic channels.

Key concepts: Transfection, Lipofectamine, HEK 293 cells, Patch clamp, Molecular biology, Cell biology, Chemistry, Gene

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